chromatography lab
![Picture](/uploads/1/4/4/7/14474898/505977.gif?391)
in this lab we preformed a chromatography technique to see the pigments in markers. during this process we measured the pigment in the markers after 5-10 minutes. after the 10 minutes we measured and recorded the lengths of the pigment that were shown.
Chromatography Of Markers Lab
By. Levi Torres
OBJECTIVE:
MATERIALS:
EXPERIMENTAL DESIGN/ PROCEDURES
RESULTS(DATA):
WATER
COLOR
DISTANCE PIGMENT
(CM)
DISTANCE WATER TRAVELED (CM)
RF VALUE =PIGMENT/WATER
RED
6.5 CM
8.25 CM
.79CM
YELLOW
5.5 CM
8.25 CM
.67 CM
BLUE
8 CM
8.25 CM
.97CM
AVERAGE
6.67 CM
8.25 CM
.81 CM
DISTANCE PIGMENT
(CM)
DISTANCE WATER TRAVELED
(CM)
RF VALUE =PIGMENT/WATER
RED
3.5 CM
4 CM
.8125 CM
YELLOW
2.75 CM
4 CM
.6875 cm
BLUE
4 cm
4 CM
1 cm
AVERAGE
3.33 cm
4CM
.83 cm
ANALYSIS:
SUMMARY
In this lab the only trend that was noticeable was that on both chromatography papers it seemed yellow was the leading pigment. then the water was the had the best result on the chromatography paper due to its solubility.
CONCLUSION:
B. The reason why I accepted my second hypothesis is because the differences in the compounds in each pigment travels at different rates in the solubility in the solvent.
3. Another error that could have occurred was that the solvent could have been spilled
3.if the solvents would have been spilled then the reaction might not of been complete due to time issues
CONTENT STANDARDS :
Cell BIOLOGY;
The fundamental life process of the plant and animals depends on a variety of chemical reactions that occur in a specialized areas of the organism’s cells.
f);students know usable energy is captured from sunlight by chloroplasts and is stored through the synthesis of sugar from carbon dioxide
Chromatography Of Markers Lab
By. Levi Torres
OBJECTIVE:
- The objective of this lab was to apply chromatography techniques with different solvents to analyze the pigments used in markers.
- HYPOTHESIS: if…then…
- I believe that alcohol will separate the pigments best
- I believe yellow will travel the furthest along the chromatography paper, followed by red then blue.
- BACKGROUND :
MATERIALS:
- 1X CHROMATOGRAPHY PAPER 1X 50mL DISTILLED WATER
- 1X 250 Ml BEAKER 1X 50mL ISOPROPYL ALCOHOL
- 1X VIS-À-VIS MARKER 1X IN,CM,MM, RULER
- 2X PETRI DISHES 1X CALCULATOR
- 2X PENCIL 1X TAPE
EXPERIMENTAL DESIGN/ PROCEDURES
- Obtain a vis-à-vis marker from the lab box # 8
- Obtain 1 strip of chromatography paper to test the marker
- In the center of the strip about 1cm from the end, place a dot of the marker to be tested. The dot should be about 1cm in diameter and dark enough to be clearly visible.
- Tape the strip to your pencil, so it hangs down.
- next place approximately 50mL of distilled water in the beaker.
- carefully insert the chromatography paper into the beaker, dotted end down. The dot must be above the level of the solvent and preferably not touching the wall of the beaker.
- allow the beaker to remain undisturbed until a good separation is obtained or until the solvent front reaches the top of the chamber. In a good separation, the colors are separated and the solvent is clearly above the top color.
- carefully remove the chromatograph from the beaker taking care not to spill any solvent.
- observe what colors were produced by the migration of pigments by the solvent and record this on your data table.
- Using a metric ruler, measure the distance the solvent traveled from the colored dot. Record this value on your data table.
- Using a pencil , draw a line at the upper edge of the migration of each pigment color. Take a ruler and measure the distance from the colored dot to the edge of the pigment band. Calculate the Rf values (Rf=distance solute (each color) moved (divided by) distance solvent moved) record these colors and the Rf value(s) on your data table.
RESULTS(DATA):
WATER
COLOR
DISTANCE PIGMENT
(CM)
DISTANCE WATER TRAVELED (CM)
RF VALUE =PIGMENT/WATER
RED
6.5 CM
8.25 CM
.79CM
YELLOW
5.5 CM
8.25 CM
.67 CM
BLUE
8 CM
8.25 CM
.97CM
AVERAGE
6.67 CM
8.25 CM
.81 CM
- ISOPROPYL ALCOHOL
DISTANCE PIGMENT
(CM)
DISTANCE WATER TRAVELED
(CM)
RF VALUE =PIGMENT/WATER
RED
3.5 CM
4 CM
.8125 CM
YELLOW
2.75 CM
4 CM
.6875 cm
BLUE
4 cm
4 CM
1 cm
AVERAGE
3.33 cm
4CM
.83 cm
ANALYSIS:
- 1.(Q) WHY DO THE PIGMENTS MOVE UP THE CHROMATOGRAPHY PAPER? EXPLAIN
- 2.(Q) WHICH SOLVENT WORKED BEST AS THE MOBILE PHASE FOR YOUR MARKER? EXPLAIN WHY.
- 3.(Q)why does the spot need to be above the level of the solvent when the chromatogram is placed into the solvent?
- 4.(Q) why does one color of pigment move further along the paper compared to the others? Explain.
- 5.(Q) what do you think would happen if we let this continue overnight?
SUMMARY
In this lab the only trend that was noticeable was that on both chromatography papers it seemed yellow was the leading pigment. then the water was the had the best result on the chromatography paper due to its solubility.
CONCLUSION:
- What I learned in this lab was that in markers there are pigmetns and that can be measured through a chromatography technique. I can use this technique on many objects like leafs, other markers, and shirts, etc. then measure there pigments to see how far they went as well.
- A. I rejected my first hypothesis that the alcohol would separate the pigment
- I reject my second hypothesis that yellow would travel furthest along the chromatography paper followed by red then blue because, it was blue then red the yellow because ,the different compounds in the sample mixture travel at different rates due to differences in solubility in the solvent. And due to differences in their attraction to the fibers in the chromatography paper
- A. The reason why I rejected my first hypothesis is because the alcohol was
B. The reason why I accepted my second hypothesis is because the differences in the compounds in each pigment travels at different rates in the solubility in the solvent.
- A.my results show that the water is the best solvent for the chromatography technique
- 1. One error that could have occurred was that I could have dipped above the dot in the solvent
3. Another error that could have occurred was that the solvent could have been spilled
- 1. If I had dipped above the dot in the solvent then the pigments would have diffused into the solvent
3.if the solvents would have been spilled then the reaction might not of been complete due to time issues
- The one thing that I have learned during this lab was that I can use the chromatography technique to test other markers. Pigment/color
CONTENT STANDARDS :
Cell BIOLOGY;
The fundamental life process of the plant and animals depends on a variety of chemical reactions that occur in a specialized areas of the organism’s cells.
f);students know usable energy is captured from sunlight by chloroplasts and is stored through the synthesis of sugar from carbon dioxide
- I.E.(investigation and experimentation)
- Scientific progress is made by asking meaningful questions and conducting careful investigations. As a basis for understanding this concept and addressing the content in the other four strands, students should develop their own questions and perform investigations. Students will:
- Safety precautions
- (F11) check labels and equipment instructions carefully. Be sure correct items are used in a proper manor .
- (F13) never taste anything or touch chemicals with hands, unless specifically instructed to do so.
- (F15) Eating or drinking in the laboratory or from laboratory equipment is not permitted.
- (F27) Keep work area clean floors and aisles should be kept clear of equipment and materials